FIG. 3.
Increased chemokine and chemokine receptor mRNA expression in the C. parvum-infected ileum. Three-day-old wild-type (WT) and β7−/− mice were infected with 106 oocysts. RNA was extracted from the ileum of neonates 4 days p.i. (+) and from their age-matched controls (−), and RT-PCRs were performed on pools of RNA samples from neonates of different litters (n = 6). Data are from one representative experiment. Number of PCR cycles for each chemokine: CCR5, 30; CCR9 and CXCR3, 32; β-actin, 25; CXCL9, CXCL10, CXCL11, and CCL4, 35.