TABLE 2.
Primer sequences used for PCR analysisa
| Target mRNA | Accession no. | Sense primer | Antisense primer | Size of PCR products (bp) |
|---|---|---|---|---|
| CD3-γ | BC027528 | 5′-TCTGGGCAACAATGCCAAAGAC-3′ | 5′-CCTCTCTGCTTGCAGTCTACC-3′ | 449 |
| CCR5 | AF022990 | 5′-ACCTTCCAGGAATTCTTTGGACT-3′ | 5′-TATACCCGATCCACAGGAGAAC-3′ | 261 |
| CCR9 | AJ131357 | 5′-GTCAGCTGTCTTGATCCTGAAG-3′ | 5′-CATAGAGAACTGGGTTCAGACAA-3′ | 320 |
| CXCR3 | AF045146 | 5′-CTGCTGGTCTCCAGAGGCCA-3′ | 5′-GTCAGTGCATCCTGGCAGCAAA-3′ | 501 |
a
The sequences of the primers used to amplify β-actin, CCL4, CXCL9, CXCL10, and CXCL11 have been previously described (11).