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Effect of IR and Cd either alone or in combination on serum estradiol levels. The values are represented as mean ± SEM of three independent experiments (n = 6). Estradiol was observed to be significantly decreased in Cd, IR, and IR + Cd group (**P < 0.01, ***P < 0.001, and ***P < 0.001) as compared to control group. IR and IR + Cd group showed significant decrease in estradiol (### P < 0.001 and ### P < 0.001) as compared to Cd group.
