Table 2.
Details of genes, accession number, primers, annealing Tm, and expected size of the PCR-amplified c-DNA.
| Gene name | GenBank accession number | Sequence of the primer Fw primer Rv primer |
Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| StAR | NM_ 31558 | 5′ AGGCAGGGGGATCTTTCTAA 3′ 5′ TGCCTGACTAGGGTTTCGTT 3′ |
56.8 | 330 |
| 17β-HSD | AF035156 | 5′ CCTCCTTCGCCACTATCAGC 3′ 5′ GGAGACAAATGAGGGCTC 3′ |
55.4 | 653 |
| CYP19A1 | NM_017085 | 5′ GGAATCCATCAAGCAGCATT 3′ 5′ TTCCACCTCCGGATACTCTG 3′ |
58 | 493 |
| β-Actin | V01217 | 5′ CCTGCTTGCTGATCCACA 3′ 5′ CTGACCGAGCGTGGCTAC 3′ |
57 | 505 |