FIG. 2.

Specific and marked reduction in the number of protein L-reactive cells in the spleens of transgenic humanized mice. Splenic cells of control (untreated) and HEL- and protein L-treated transgenic mice were depleted of erythrocytes by using Tris-buffered ammonium chloride and stained with cell surface markers. (A) Total number of splenic cells counted by using a Malassez cell; (B) HuIgM⁺ B220⁺ cells as detected by PE-labeled anti-HuIgM and CyChrome-labeled anti-mouse B220 MAbs; (C) B220⁺ protein L⁺ cells as detected by FITC-labeled anti-mouse B220 MAb and biotinylated protein L, followed by treatment with CyChrome-labeled streptavidin.