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. 2014 Spring;13(2):551–559.

Table 1.

Antioxidant performance and total phenolic contents of the extracts/fractions from A.biennis.

Sample Extraction/ fractionation yield (g) EC 50 (µg/ mL)
TPC
(mg GAE /g)
DPPH assay FIC assay BCB assay
PE 42.20 1314.86 ±37.01 258.17 ± 27.71 283.37 ± 4.31 0.19 ± 0.33
DCM 57.80 452 ± 0.02 343.27 ± 10.13 313.18 ±6.25 8.65 ± 2.10
EA 3.70 74.20 ± 1.17 578.71 ± 37.17 212.20 ± 6.71 66.95 ± 0.56
EtOH 11.40 67.39 ± 2.72 269.85 ± 10.69 189.63 ± 7.10 107.02 ± 4.23
EtOH/Wt 79.50 44.05 ± 0.42 132.47 ± 5.33 132.63 ± 4.43 122.21 ± 1.85
Fr. A 19.35 214.41 ± 2.74 22.79 ± 1.34 58.44 ± 0.72 12.33 ± 0.48
Fr. B 6.89 51.50 ± 0.19 30.95 ± 1.55 71.86 ± 2.97 69.15 ± 2.25
Fr.C 2.15 22.01 ± 0.18 54.55 ± 4.97 53.27 ± 16.79 289.13 ± 4.20
Fr. D 4.16 14.98 ± 0.14 66.07 ± 3.09 17.55 ± 3.01 338.61 ± 6.67
Fr. E 3.46 23.64 ± 0.69 115.66 ± 2.09 18.56 ±5.30 318.16 ± 3.59
Fr. F 1.88 27.69 ± 0.45 242.42 ± 7.83 158.07± 1.96 62.67 ± 1.92
Fr. G 1.35 194.74 ± 9.16 180.47± 2.69 245.02 ± 13.46 17.48 ± 10.83
BHT --- 4.88±0.57 --- 0.458±0.07 ---
Vit C --- 3.66±0.28 --- --- ---
EDTA --- --- 18.00 ± 3.02 --- ---
Quercetin --- --- 87.24 ± 3.94 --- ---