FIGURE 2.

mRNA expression levels of CFH, CFHR-B and CFHR-C analysis of various organs of NOD mice without and with diabetes. Eighteen-week-old, but not 4-week-old NOD mice were susceptible to the DM. Total RNA was extracted from brain, liver, lung, heart, spleen, kidney, knee joint and skin from NOD mice with and without DM. All RNA samples were analyzed in duplicate by qRT-PCR. The mRNA for CFH and CFHR-B were universally present in liver, brain, lung, heart, spleen, kidney, knee joint and skin of mice with and without DM. However, CFHR-C mRNA was universally absent in all organs. A. CFH mRNA levels in various organs of NOD mice with no DM. B. CFHR-B mRNA level in various organs of NOD mice with no DM. b. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B in BR, LG, HT, SPL, KID, and KJ. C. No detection of CFHR-C mRNA in various organs including liver of NOD mice with no DM. c. A small histogram embedded in the main histogram showing universal absence of mRNA for CFHR-C in BR, LG, HT, SPL, KID, KJ and SK in mice without DM. D. CFH mRNA levels in various organs of NOD mice with DM was detectable in all organs. E. CFHR-B mRNA levels in various organs of NOD mice with DM. e. A small histogram embedded in the main histogram showing presence of detectable levels of mRNAs for CFHR-B in BR, LG, HT, SPL, KID, KJ and SK in mice with DM. F. No detection of CFHR-C mRNA in various organs including liver of NOD mice with DM. f. A small histogram embedded in the main histogram showing complete absence of mRNAs for CFHRC in BR, LG, HT, SPL, KID, KJ and SK in mice with DM. All data presented in these graphs, as Mean ± SEM, were from NOD mice with no DM (n = 5) and NOD mice with DM (n = 5). The q-RTPCR was repeated two times. BR = brain, LG = lung, HT = heart, SPL = spleen, KID = kidney, KJ = knee joint and SK = skin. *p-value < 0.05 compared to liver