FIGURE 4.

mRNA expression levels of CFH, CFHR-B and CFHR-C analysis of various organs in mice with and without CAIA. CAIA was induced in C57BL/6 mice as described in Materials and Methods. All mice with CAIA were sacrificed at day 10. Age-matched WT mice were also sacrificed in parallel at day 10 without the induction of CAIA. Total RNA was extracted from brain, liver, lung, heart, spleen, kidney, knee joint and skin from WT mice with and without arthritis. A. CFH mRNA various organs of mice with no CAIA. B. CFHR-B mRNA in various organs of mice with no CAIA. b. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B in BR, LG, HT, SPL, KID, and KJ but not in SK. C. CFHR-C mRNA in various organs of mice with no CAIA. c. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B only in SK. D. CFH mRNA in various organs of mice with CAIA. E. CFHR-B mRNA in various organs of mice with CAIA. e. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B in BR, LG, HT, SPL, KID, KJ and SK. F. CFHR-C mRNA in various organs of mice with CAIA. f. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B in SPL, KJ and SK. All RNA samples were analyzed in duplicate by qRT-PCR. All data presented in these graphs, as Mean ± SEM, were from WT mice with no CAIA (n = 4) and WT mice with CAIA (n = 5). BR = brain, LG = lung, HT = heart, SPL = spleen, KID = kidney, KJ = knee joint and SK = skin. *p-value < 0.05 compared to liver