Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Immunol. 2014 Jul 15;62(1):235–248. doi: 10.1016/j.molimm.2014.06.028

FIGURE 5.

FIGURE 5

mRNA expression levels of CFH, CFHR-B and CFHR-C analysis of various organs in mice with and without SLE. At 23 weeks of age vs. 9 weeks MRL-lpr/lpr showed clinical signs of lupus. Total RNA was extracted from brain, liver, lung, heart, spleen, kidney, knee joint and skin from MRL-lpr/lpr with and without lupus. All RNA samples were analyzed in duplicate by qRT-PCR. The mRNA for CFHR-C was absent not only in the liver of mice with and without lupus, but it was absent universally in brain, lung, heart, spleen, kidney, knee joint and skin. A. fH mRNA in various organs of MRL-lpr/lpr mice with no lupus. B. CFHR-B mRNA in various organs of MRL-lpr/lpr mice with no lupus. The mRNA was present in all organs, but in skin it was very low. b. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B in BR, LG, HT, SPL, KID, KJ and SK. C. Undetectable levels of CFHR-C mRNA in all organs of MRL-lpr/lpr mice with no lupus. D. CFH mRNA in various organs of MRL-lpr/lpr mice with lupus. It was detectable in all organs except in skin it was low. E. CFHRB mRNA in various organs of MRL-lpr/lpr mice with lupus. e. A small histogram embedded in the main histogram shows detectable mRNA levels of CFHR-B in BR, LG, HT, SPL, KID, KJ and SK. F. Undetectable levels of CFHR-C mRNA in various organs of FH mRNA in various organs of MRL-lpr/lpr mice with lupus. All data presented in these graphs, as Mean ± SEM, were from MRL-lpr/lpr mice with no lupus (n = 5) and MRL-lpr/lpr mice with lupus (n = 5). These experiments were repeated three times. BR = brain, LG = lung, HT = heart, SPL = spleen, KID = kidney, KJ = knee joint and SK = Skin. *p-value < 0.05 compared to liver.