FIGURE 6.
Analysis of the mRNA expression levels of CFH, CFHR-B and CFHR-C in various organs of NZB/NZW mice, with and without SLE, and in LG/J mice without SLE. At ~54 weeks of age NZB/NZW showed clinical signs of lupus. Total RNA was extracted from liver of NZB/NZW with (54 weeks) and without lupus (9 weeks). Liver was also obtained from LG/J mice without lupus (age 9 weeks). All RNA samples were analyzed in duplicate by qRT-PCR. The mRNA for CFH and CFHR-B were present in liver of NZB/NZW and LG/J mice with and without lupus. The mRNA for CFHR-C was totally absent in liver of both NZB/NZW and LG/J mice regardless of disease.