Table 1.

Primers and probes for quantitative RT-PCR used to determine the levels of mouse CFH, CFHR-B and CFHR-C mRNAs

Gene name: CFH*
Forward Primer (5′-3′)	GTGGGCATCCCGGAGAC
Reverse Primer (5′-3′)	CACCAAACTCAAATTGAGATCCAA
Probe (5′-3′)	CACCCTTTGGGTCCTTTAGGCTGGC
Product size	69 bp
Gene name: CFHR-B
Forward Primer (5′-3′)	AAAATGGTCAAGACACAATGACATG
Reverse Primer (5′-3′)	TTGTTGAGTTGATACGGATGCAT
Probe (5′-3′)	AGAATGGCTGGTCCC
Product size	78 bp
Gene name: CFHR-C
Forward Primer (5′-3′)	TCCCAGCCCCCTACAATAGA
Reverse Primer (5′-3′)	TCATGACTGCTGAACTCAATGGA
Probe (5′-3′)	ACCCAGAACATCTGAAAA
Product size	89 bp

^*primers for mouse CFH have been designed using exon #3 except for cfhTg/mCfh^−/− mice. The designs for CFHR-B and CFHR-B primers and probes have been explained in the Methods section. Primers and probes were purchased from Life Technologies, Grand Island, NY