Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biomaterials. 2014 Aug 22;35(35):9546–9553. doi: 10.1016/j.biomaterials.2014.08.004

Figure 3.

Figure 3

The expression of sigma-2 receptor in head and neck cancer and its effect on the cellular uptake of MBA-Pc 4-TRN. The expression of sigma-2 receptor in normal tissue (A) and human head and neck cancer tissue (B). Representative images shown. Sigma-2 receptor positive tissue areas were detected with immunohistochemistry and quantified as percent of total tissue and expressed as fold of normal tissue (mean ± SE, n >30, # p< 0.001) (C); The expression of sigma-2 receptor in UMSCC22A cell line detected by Western immunoblotting (D); Confocal images of UMSC22A cells treated with Pc 4 or Pc 4 loaded TRN (E). Cells were cultured with Pc 4 or Pc 4 loaded TRN (red) for 20 h. Cell nuclei were stained with DAPI (blue). Images from left to right were taken from DAPI, Pc 4, transmittance, and the overlay of the previous three channels; (F) Intracellular amount of Pc 4 for cells treated with Pc 4-TRNs containing different MBA densities (mean ± SD, n = 3).