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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: J Immunol. 2014 Aug 15;193(6):2843–2849. doi: 10.4049/jimmunol.1401102

FIGURE 1.

FIGURE 1

Integrin αvβ8 is expressed by Foxp3+ Tregs. (A) Sorted CD4+Foxp3+(GFP+) and CD4+Foxp3− (GFP−) were measured for Itgb8 and Foxp3 message as measured by qRT-PCR. Data is expressed relative to CD4+Foxp3− cells. (B and C) Sorted CD4+CD25+ and CD4+CD25− from CD4 conditional knockouts of Tgfb1 and Lrrc32 and their CRE-littermate controls were measured for Itgb8 message. Data is expressed relative to wild-type CD4+CD25− cells. (D) Sorted CD4+Foxp3+(GFP+) and CD4+Foxp3-(GFP-) from spleen, peripheral lymph nodes, or mesenteric lymph nodes were measured for Itgb8 message as measured by qRT-PCR. (E) Sorted CD4+Foxp3(GFP) − and CD4+Foxp3(GFP)+ (fresh or after overnight stimulation with plate-bound anti-CD3+ IL-2), CD11b+CD103- and CD11b−CD103+ DCs from the mesenteric LNs, and CD11b+ or CD11b− DCs were measured for Itgb8 message. DCs were first gated on Thy1.2-CD19-CD11c+I-Ab-hi cells.