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. 2004 Jun;72(6):3195–3203. doi: 10.1128/IAI.72.6.3195-3203.2004

FIG. 4.

FIG. 4.

Western blot reactivities of sera from B. burgdorferi-infected MyD88−/− and WT mice. Sera from individual mice infected for 28 days were assessed for reactivity to B. burgdorferi proteins by immunoblotting of B. burgdorferi lysates. (A) IgM reactivities of WT (lanes 1 to 5) and MyD88−/− mouse sera (lanes 6 to 10). Arrows mark the locations of OspC and DbpA, which were assessed by the use of antisera for these proteins. (B) IgG reactivities of WT (lanes 3 to 7) and MyD88−/− mouse sera (lanes 8 to 12). Lanes 1 and 2 depict the locations of OspA and OspC, respectively, as assessed by the use of OspA MAb VIIIC3.78 and an antiserum to recombinant OspC. The ∼30-kDa protein bound by antibodies in both WT and MyD88−/− sera migrates below OspA. The unlabeled arrow marks the location of a protein targeted by the WT but not the MyD88−/− immune serum. (C) Isotype-specific immunoblot of B. burgdorferi lysates using sera pooled from individual WT and MyD88−/− mice. KO, MyD88−/− mice.