FIG. 5.

Splenic CD4⁺-T-cell production of IL-4 and IFN-γ. CD4⁺ T cells were purified from the spleens of infected WT and MyD88^−/− mice and stimulated in vitro with B. burgdorferi lysates. After 72 h, supernatants were harvested and the amounts of IL-4 (A) and IFN-γ (B) were analyzed by cytokine-specific ELISAs. The results are reported as the means of triplicate samples ± standard errors of the means (A) or of duplicate samples (B). The minimum level of cytokine detection was 30 pg/ml for IL-4 and 30 pg/ml for IFN-γ.