Table I.
Absolute numbers of lymphocytes and progenitors in the spleen and thymus of WT and CD11a−/−mice.
| A. Spleen | |||
|---|---|---|---|
| Population | WT | CD11a−/− | p value |
| CD3 | 22.70±0.8 | 16.82±1.9 | 0.03 |
| CD3+TCRγδ−CD4+ | 13.45±0.6 | 6.31±0.9 | 0.006 |
| CD3+TCRγδ−CD8+ | 8.39±0.3 | 7.91±0.9 | NS |
| TCRγδ | 0.56±0.0 | 2.72±0.2 | <0.0001 |
| NK | 3.37±0.2 | 3.19±0.2 | NS |
| CD19 | 45.40±3.7 | 22.09±1.2 | 0.001 |
| B. Thymus | |||
|---|---|---|---|
| Population | WT | CD11a−/− | p value |
| CD4−CD8− (DN) | 3.088±0.4 | 1.673±0.8 | 0.03 |
| DN1 | 0.150±0.03 | 0.0375±0.8 | 0.005 |
| DN2 | 0.0175±0.01 | 0.0150±0.8 | NS |
| DN3 | 0.975±0.2 | 1.028±0.8 | NS |
| DN4 | 1.950±0.29 | 0.5925±0.08 | 0.004 |
| ETP | 0.006±0.001 | 0.002±0.0003 | 0.02 |
| c-kit+ DN2 | 0.015±0.005 | 0.006±0.0006 | 0.09 |
| DP | 67.87±10.76 | 31.87±3.697 | 0.0195 |
| CD4 SP | 5.12±0.73 | 1.31±0.23 | 0.0015 |
| CD8 SP | 1.31±0.13 | 0.35±0.07 | 0.0006 |
| TCRγδ | 0.136±0.014 | 0.184±0.028 | NS |
(A) Total cell numbers (x106) and statistical comparison of indicated lymphocyte subsets within the spleen of WT and CD11a−/− mice. (B) Total cell numbers of thymocytes from WT and CD11a−/− mice. DN (CD3−TCRγδ−/−CD4−CD8−) cells were separated based on CD44 and CD25 expression to identify DN1-4 subsets or by c-kit and CD25 expression to identify ETPs and c-kit+DN2 subsets. CD3+TCRγδ−/− cells were further subdivided into CD4+ (CD4 SP) and CD8+ (CD8 SP) thymocytes. p values greater than 0.05 are noted as NS, not significant. Data is inclusive of 5 mice per group. Mice ranged in age from 8–12 weeks. Two individual experiments were performed.