Table 1.
Diagnostic accuracy results of individual studies using the ELISA method, including the commercial kits Panbio Dengue Early ELISA, Dengue NS1 Ag ELISA, and Platelia Dengue NS1 Ag-ELISA
| Author (year) | Geography | Design | Size | Confirmed testsa | TP | FP | FN | TN | Sensitivity % (95% CI) | Specificity % (95% CI) | PPV % | NPV % | QUADAS |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ramirez et al. (2009)b | South America (Venezuela) | Retrospective | 147 | 1 | 53 | 2 | 34 | 58 | 61 (50–71) | 97 (98–100) | 96 | 63 | 8 |
| Watthanaworawit et al. (2010)b | Southeast Asia (Thailand/Myanmar) | Prospective | 152 | 3 | 39 | 0 | 33 | 90 | 54 (42–66) | 100 (96–100) | 100 | 73 | 10 |
| Osorio et al. (2010)b | Southeast Asia (Colombia) [Au?16] | Retrospective | 400 | 1 | 155 | 10 | 63 | 82 | 71 (65–77) | 89 (81–95) | 94 | 57 | 11 |
| Lima et al. (2010)b | South America (Brazil) | Prospective | 550 | 1 | 159 | 0 | 61 | 230 | 72 (66–78) | 100 (88–100) | 100 | 79 | 9 |
| Pok et al. (2010)b | Europe (Singapore) [Au?16] | Prospective | 209 | 2 | 73 | 0 | 36 | 100 | 67 (57–76) | 100 (96–100) | 100 | 74 | 8 |
| Fry et al. (2011)b | Southeast Asia (Vietnam) | Prospective | 298 | 4 | 137 | 4 | 61 | 96 | 69 (62–76) | 96 (90–99) | 97 | 61 | 9 |
| Blacksell et al. (2012)b | Southeast Asia (Thailand), Asia (Sri Lanka) | Retrospective | 387 | 2 | 107 | 12 | 132 | 136 | 45 (38–51) | 92 (86–96) | 90 | 51 | 10 |
| Osorio et al. (2010)c | Southeast Asia (Colombia) [Au?16] | Retrospective | 400 | 1 | 150 | 5 | 68 | 87 | 69 (62–75) | 95 (88–96) | 97 | 56 | 11 |
| Wang et al. (2010)c | Southeast Asia (Malaya) | Prospective | 244 | 1 | 142 | 1 | 43 | 58 | 77 (70–83) | 98 (91–100) | 99 | 57 | 9 |
| Blacksell et al. (2012)c | Southeast Asia(Thailand), Asia (Sri Lanka) | Retrospective | 387 | 2 | 132 | 2 | 107 | 146 | 55 (49–62) | 99 (95–100) | 99 | 58 | 10 |
| Kumarasamy et al. (2007)d | Southeast Asia (Malaysia) | Retrospective | 567 | 2 | 199 | 0 | 4 | 354 | 96 (95–99) | 100 (96–100) | 100 | 99 | 8 |
| Lapphra et al. (2008)d | Southeast Asia (Thailand) | Prospective | 235 | 1 | 108 | 1 | 63 | 63 | 63 (55–70) | 96 (92–100) | 99 | 50 | 11 |
| Ramirez et al. (2009)d | South America (Venezuela) | Retrospective | 143 | 1 | 62 | 5 | 25 | 55 | 71 (61–80) | 92 (82–97) | 93 | 69 | 8 |
| Phuong et al. (2009)d | Southeast Asia (Vietnam) | Prospective | 459 | 2 | 20 | 2 | 34 | 403 | 37 (24–52) | 100 (96–100) | 91 | 92 | 8 |
| Osorio et al. (2010)d | Southeast Asia (Colombia) [Au?16] | Retrospective | 400 | 1 | 150 | 7 | 62 | 84 | 71 (64–77) | 92 (85–97) | 96 | 56 | 11 |
| Lima et al. (2010)d | South America (Brazil) | Prospective | 550 | 1 | 184 | 3 | 36 | 227 | 84 (78–88) | 99 (85–97) | 98 | 86 | 10 |
| Pok et al. (2010)d | Europe (Singapore) [Au?16] | Prospective | 209 | 2 | 89 | 0 | 20 | 100 | 82 (73–88) | 100 (96–100) | 100 | 45 | 8 |
| Duong et al.(2011)d | Southeast Asia (Cambodia) | Prospective | 359 | 5 | 150 | 0 | 110 | 79 | 58 (51–64) | 100 (95–100) | 100 | 42 | 8 |
| Najioullah et al. (2011)d | North America (Martinique) | Prospective | 537 | 3 | 156 | 0 | 99 | 271 | 61 (55–67) | 100 (96–100) | 100 | 73 | 10 |
| Kassim et al. (2011)d | Southeast Asia (Malaysia) | Retrospective | 208 | 3 | 60 | 7 | 77 | 64 | 44 (35–53) | 90 (81–96) | 90 | 45 | 7 |
| Blacksell et al. (2012)d | Southeast Asia(Thailand), Asia (Sri Lanka) | Retrospective | 387 | 2 | 107 | 12 | 132 | 136 | 45 (38–51) | 92 (86–96) | 90 | 51 | 9 |
TP, true positive; FP, false positive; FN, false negative; TN, true negative; CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; QUADAS, Quality Assessment for Diagnostic Accuracy Studies.
Confirmed tests: (1) serological, nucleotide, or viral culture test; (2) serological or nucleotide test; (3) serological test; (4) nucleotide test; (5) serological, NS1 detection, nucleotide, or viral culture test.
Statistics retrieved from studies using the Panbio Dengue Early ELISA Kit.
Statistics retrieved from studies using the Dengue NS1 Ag ELISA Kit.
Statistics retrieved from studies usins the Platelia Densue NS1 As-ELISA