Figure 1. IFNαR signaling in T cells is required for immune dysfunction in Trex1^−/− mice.

a) Top: percent weight change in Rag2^−/−Trex1^+/−(“Trex1^+/−“, black squares) and Rag2^−/−Trex1^−/−(“Trex1^−/−“, open squares) mice at various time points after co-transfer of WT CD45.2⁺CD4⁺CD25⁺ Treg and WT CD45.1⁺CD4⁺Foxp3^GFP−CD45RB^hi T_Naive cells. Bottom: absolute number of intraepithelial (IEL) and lamina propria lymphocytes (LPL) in the colons of Rag2^−/−Trex1^+/− (black) and Rag2^−/−Trex1^−/−(checkered) recipient mice at time of sacrifice. b) Representative H&E staining of cross-sections of intermediate to distal colon from Rag2^−/−Trex1^+/− and Rag2^−/−Trex1^−/− recipients of WT Treg and WT T_Naive cells at time of sacrifice. c) Top: percent weight change in Rag2^−/−Trex1^+/−(“Trex1^+/−“, black squares) and Rag2^−/−Trex1^−/−(“Trex1^−/−“, open squares) mice at various time points after co-transfer of Ifnar1^−/− CD45.2⁺CD4⁺CD25⁺ Treg and Ifnar1^−/− CD45.1⁺CD4⁺Foxp3^GFP−CD45RB^hi T_Naive cells. Bottom: absolute number of intraepithelial (IEL) and lamina propria lymphocytes (LPL) in the colons of Rag2^−/−Trex1^+/− (black) and Rag2^−/−Trex1^−/−(checkered) recipient mice at time of sacrifice. d) Representative H&E staining of cross-sections of intermediate to distal colon from Rag2^−/−Trex1^+/− and Rag2^−/−Trex1^−/− recipients of Ifnar1^−/− Treg and Ifnar1^−/− T_Naive cells at time of sacrifice. Statistical significance was determined using unpaired two-tailed Student’s t-test. Data are representative of two independent experiments with 3–4 mice per group. *, p<0.05; **, p<0.005; ***, p<0.0001.