Figure 1. Serum stability of anionic and cationic liposomes.
Aggregation of Ad, Lec_Ad, and DOTAP_Ad was monitored over 24 hrs in 50% human serum by measurement of absorbance at 560 nm at 37 °C. All samples had a final viral particle concentration of 2.5×1010vp/ml, where Lec_Ad and DOTAP_Ad complexes had a final lipid concentration of 1.4mg lipid/ml and 1.3mg lipid/ml, respectively. Aggregation of particles in serum is observed for DOTAP while lecithin liposomes were stable over time.