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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biomaterials. 2014 Aug 22;35(35):9554–9561. doi: 10.1016/j.biomaterials.2014.08.010

Figure 2. Transfection efficiency at a wide range of MOIs.

Figure 2

A549 cells were transfected with Ad, lecithin-Ad (Lec_Ad), and DOTAP_Ad, before and after immunoprecipitation (IP) at MOIs 2.2, 4.3, 10.75, 21.5, and 43 pfu/cell. Lec_Ad +IP (purple) had the highest transfection efficiency at a wide-range of MOIs in comparison to naked Ad, DOTAP_Ad, and Lec_Ad before IP most likely due to reduction of size and homogenization after immunoprecipitation. DOTAP_Ad +IP (black) showed no signal suggesting that Ad was not fully encapsulated or IP is disrupting liposome layers due to charge interactions. Ad+IP (red) and DOTAP_Ad +IP showed no signal and data points are overlaid.