Figure 6. Homeostasis of Tregs during SEB-induced toxic shock syndrome in HLA-DR3 transgenic mice.
HLA-DR3 transgenic mice were left untreated or injected with IL-2, anti-IL-2 immune complexes on days 0, 1 and 2 to expand Tregs (3xIL2C). On day 5, mice were challenged with SEB (50 µg/mouse) or PBS and killed 3 days later. The percentage of CD25+FoxP3+ T cells in the CD4+ gated splenocytes were determined by flow cytometry and their absolute numbers were deduced. Each bar represents mean±SE of data obtained from 6–8 mice in each group. * p<0.05 compared to naïve mice. # p<0.05 compared to IL2C group.