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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: J Immunol. 2014 Aug 4;193(6):2919–2930. doi: 10.4049/jimmunol.1400980

Figure 7. Expression profile of GITR on Tregs and Tconv cells in HLA-DR3 transgenic mice.

Figure 7

HLA-DR3 transgenic mice were left untreated or injected with IL-2, anti-IL-2 immune complexes on days 0, 1 and 2 to expand Tregs. On day 5, mice were challenged with SEB (50 µg/mouse) or PBS. Mice were killed 24 (day 6) or 48 hours (day 7) later, splenocytes isolated and stained with indicated antibodies. (A) Representative dot plots depicting distribution of CD4+FoxP3+ and CD4+FoxP3 T cells in different groups of mice (B) Histogram overlays depicting expression of GITR on the cells gated as above (C) Bar chart depicting percentages of GITR+ cells within the indicated gates and (D) Bar chart depicting median fluorescent intensity of GITR expression on cells within the indicated gates. Each bar represents mean±SE of data obtained from 3 mice in each group. * p<0.05 compared to naïve mice.