Table.
Cell subpopulations* in normal mammary glands and tumors extracted from MMTV-PyMT mice.
| % of CD45 positive cell | % of CD45 negative cell | |||
|---|---|---|---|---|
| Normal MG | MMTV- PyMT tumor | Normal MG | MMTV- PyMT tumor | |
| TDMMC (CD45+CD11b+CD11c+F4/80+MHCII+Gr1−) | 14.9±0.7 | 76.5±5.4 | - | - |
| MDSC (CD45+CD11b+CD11c−F4/80−MHCII−Gr1+) | 2.1±0.09 | 9.2±1.7 | - | - |
| T cells (CD45+CD3+) | 73.3±3.8 | 11.7±0.6 | - | - |
| Fibroblasts (CD45−CD326−CD140a+) | - | - | 81.7.0±6.8 | 11.4±1.3 |
| Epithelial/Cancer cells (CD45−CD326+CD140a−) | - | - | 8.9±1.5 | 75.9±6.9 |
| Endothelial (CD45−CD326−CD31+CD102+) | - | - | 7.3±0.8 | 1.8±0.4 |
Single cell suspensions obtained from normal mammary gland (MG) or MMTV-PyMT tumors were initially gated as DAPI-negative population to exclude dead cells. Then, viable cells were adjusted to singlets using FSC-A/ FSC-H dot plot. Viable singlet cell population was then used to identify different cell subpopulation based on cell surface markers indicated in the table. Data are presented as mean ± SEM of five animals in each group.
Mean values of percentage of CD45+ cells were 49.6±5.3% and 18.3±7.1% in normal MG and MMTV-PyMT tumor, respectively. Total cell numbers in cell suspensions from normal mammary glands and tumors were 1.1±0.3 × 107 cell and 9.8±2.6 × 107 cell per g of tissue, respectively.