Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Virology. 2014 Jul 24;0:118–133. doi: 10.1016/j.virol.2014.06.035

Figure 6. KSHV colocalizes on cell surface domains with CD98, αVβ5 and α3β1 integrins, and the lipid raft marker cholera toxin B.

Figure 6

HT1080 cells were incubated with DNP-KSHV (red) and antibodies to either A) CD98, C) α3β1, D) αVβ5, (E) biotinylated cholera toxin B (bio-CTB), a marker of lipid rafts [36] or F) caveolin-1, a marker of caveolae. The fluorescent signals were sequentially enhanced with TSA 488 (antibodies to cellular proteins and cholera toxin B, green) and TSA 647 (anti-DNP, red), and the merged images are shown (colocalized pixels, white). Supranuclear domains are indicated (yellow dashed outline). Manders colocalization coefficients M1 and M2 were A) CD98: 0.70/0.90; C) α3β1: 0.955/0.726; D) αVβ5: 0.948/0.68; E) bio-CTB: 0.82/0.716 and F) caveolin-1: 0.039/0.42, where M1 indicates the percentage of DNP-KSHV detected that colocalizes with the cellular marker, and M2 indicates the percentage of the cellular marker detected that colocalizes with DNP-KSHV. Fluorescent intensities for CD98 and KSHV along the arrow in panel A are shown in B. Bar=10μm