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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Virology. 2014 Jul 24;0:118–133. doi: 10.1016/j.virol.2014.06.035

Figure 9. HSG epithelial cells are resistant to KSHV infection and lack KSHV-binding domains.

Figure 9

A) Vero or B) human salivary gland (HSG) epithelial cells were incubated with gradient purified KSHV (~1,000 genomes/cell) for 3 hours, washed, and incubated for 24 hours. The cells were fixed and reacted with an antibody to KSHV LANA to detect latent infection, and fluorescence was enhanced using TSA 488 (green). Nuclei were stained with TO PRO-3 (blue). C) LANA expression was quantitated by counting fluorescent pixels per nucleus. Vero cells (median = 2367 pixels/nucleus); HSG cells (median = 0.0 pixels/nucleus) (P<0.0001; Mann-Whitney U test). D) DNP-KSHV was incubated with HSG cells or control HT1080 cells (inset) and bound virus was detected using anti-DNP and TSA 488 (green). Bar = 20 μm