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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Immunol. 2014 Jun 28;62(1):96–103. doi: 10.1016/j.molimm.2014.06.013

Figure 1.

Figure 1

Differential expression of HVEM and LTβR onHeLa and HT-29 cells is associated with altered TRAF2 stability.A. Expression of HVEM and LTβR on different cell lines, measured by flow cytometric bead assay as described in the Methods. Experiment was repeated three times and data averaged.HeLa and HT-29 cells express similar levels of LTβR but different levels of HVEM. B. HeLa and HT-29 cells degrade IκBα with similar kinetics after stimulation with LIGHT. TNF-α was used as a positive control. C. Transient activation of JNK in HeLa and HT-29 cells is observed in response to LIGHT stimulation. TNF-α was used as a positive control. D. TRAF2 stability in HT-29 cells after LIGHT stimulation is increased relative to that in HeLa cells. B-D show representative blots from experiments in duplicate.