Figure 2. Rapid mobilization of effector Th1 cells during influenza infection.

B10.BR mice were s.c. immunized with Hb_64–76, PCC_88–104, or PCC_103K peptides, challenged 7 days later with WSN-MCC_88–104 influenza virus, and analyzed on day 4 postinfection. (A) Total number of Ag-specific CD4 T cells (Dapi⁻B220⁻CD8a⁻CD11b⁻Vα11⁺Vβ3⁺CD44^hi) in BALF (left) and lungs (right). (B) Total number of IFN-γ⁺ Ag-specific CD4 T cells in lungs measured by ICS. Means ± SEM for at least three animals are shown. *p ≤ 0.05, **p ≤ 0.01 (unpaired Student t test). Data shown are derived from at least three independent experiments (n ≥ 3 mice per group). (C) IL-2, IFN-γ, and IL-10 concentrations in BALF of infected (n≥9) or naïve (n=14) mice measured by multiplex.