FIG. 4.
r27 is recognized by TLR2. (A) RAW 264.7 cells (5 × 105/well) were stimulated with various concentration of r27 or r27ΔSP in the presence of polymyxin B. After 24 h, culture supernatants were collected from the wells, and IL-12 levels were measured by ELISA. (B) Thioglycolate-elicited peritoneal macrophages from C3HEB/FeJ and C3H/HeJ mice were stimulated with LPS (10 ng/ml) or r27 (10 μg/ml), supernatants were collected after 24 h, and IL-12 secretion was measured by ELISA. (C) CHO/CD14 and CHO/CD14/TLR2 cells (5 × 105/well) were stimulated with r27 or r27ΔSP (5 μg/ml) for 24 h. After this period, cells were collected, labeled with fluorescein isothiocyanate-conjugated anti-human CD25, and then analyzed by FACS. Similar results were obtained in four independent experiments.