Figure 3. NKG2D^TR downregulates surface expression of NKG2D^FL.

(A) Human CD8⁺ T cells were untransduced, transduced with vector (GFP), or with different combinations of retroviruses encoding NKG2D^FL-Flag, NKG2D^TR-myc=GFP, NKG2D^TR-myc, and DAP10^HA=GFP, and analyzed for NKG2D^FL surface expression. NKG2D^TR-myc=GFP was used in double transduction of NKG2D^TR and NKG2D^FL. Filled histograms and solid lines represent cells stained with IgG control antibody and anti-NKG2D, respectively. One representative of n = 6 independent experiments is shown. (B) Human CD8⁺ T cell cells were transduced with vectors expressing either scrambled shRNA (solid line) or NKG2D^TR shRNA (dotted line) and analyzed for NKG2D^FL surface expression. The filled histograms represent cells stained with IgG control antibody. One representative of n = 3 donors is shown. (C) Human CD8⁺ T cells were transduced with GFP alone or with NKG2D^TR-Myc. Surface and intracellular NKG2D was detected by flow cytometry. Filled histograms and solid lines represent cells stained with IgG control antibody and anti-NKG2D, respectively. One representative of n = 3 experiments is shown.