Figure 4. NKG2D^TR does not change total NKG2D^FL expression but blocks the interaction of NKG2D^FL with DAP10.

(A). Human CD8⁺ T cells were transduced with either vector (GFP alone) or NKG2D^TR. Total cell lysates were blotted for NKG2D, γ-tubulin (loading control), or DAP10. Cell lysates were also immunoprecipitated (IP) with anti-NKG2D^FL antibody and immunoblotted (IB) with antibody against NKG2D^FL or anti-DAP10. One representative of n = 3 independent experiments is shown. (B) Total cell lysates from CD8⁺ T cells untransduced (left lane), transduced with scrambled shRNA (middle lane), NKG2D^TR shRNA vector (right lane) were blotted for NKG2D, γ-tubulin (loading control), or DAP10. Cell lysates were also immunoprecipitated (IP) with anti-NKG2D^FL antibody and immunoblotted (IB) with antibody against NKG2D^FL or anti-DAP10. One representative of n = 3 donors is shown.