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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Immunol. 2014 Jun 25;62(1):54–62. doi: 10.1016/j.molimm.2014.05.019

Figure 2. Cytokines induce production of TNFα in 832/13 cells and TNF-α enhances surface expression of Cd11b, but not Cd11c, in bone-marrow derived neutrophils.

Figure 2

A, B. 832/13 cells were treated with IL-1β (1 ng/mL), IFN-γ (100 U/mL) or the combination for 3, 6 or 12 h. TNFα secretion into the media (A) and cellular TNFα content (B) were quantified by ELISA and normalized to total protein. ***, P < 0.001 vs. NT, **, P < 0.01 (grey bars vs. NT), n.s. = not significant. ELISA assays were performed on three separate occasions. Data are expressed as means ± SEM. C,D. Isolated murine bone marrow neutrophils were exposed to 1ng/ml of TNFα for 30min (TNF) or media alone (Con). Cells were stained with antibodies to CD11c APC or CD11b PE and analyzed by flowcytometry. Results shown are representative of three biological replicates.