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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Immunol. 2014 Jun 25;62(1):54–62. doi: 10.1016/j.molimm.2014.05.019

Figure 3. IL-1β-dependent stimulation of TNFα requires IκKβ.

Figure 3

A. 832/13 cells were pre-treated for 1 h with either DMSO (vehicle control) or the indicated concentrations of the IκKβ inhibitor TPCA. Cells were subsequently treated for 3 h with 1 ng/mL IL-1β. Relative TNFα mRNA abundance was normalized to RS9. **, P < 0.01 vs. DMSO, *, P < 0.01 vs. DMSO. B. 832/13 cells were transfected with siRNA duplexes targeting either a scrambled control sequence (siScramble) or IκKβ (siIκKβ). After 48 h exposure to siRNA, cells were harvested and mRNA levels of IκKβ were quantified. **, P <0 .01. C. 832/13 cells were transfected with siScramble (siScr), siIκKβ or siIκKβ or siIκKα. After 48 h culture with the indicated siRNA duplexes, cells were harvested and an immunoblot performed to determine the cellular abundance of IκKβ; Actin was used as the loading control. The immunoblot shown is representative of two independent experiments. D. 832/13 cells were transfected with siScramble and siIκKβ duplexes. After 48 h, cells were stimulated with 1 ng/mL IL-1β for 3 h. TNFα mRNA level was quantified and normalized to RS9. **, P < 0.01. For mRNA experiments, three individual replicates were generated and expressed as means ± SEM.