Table 4.

Pathogenesis of ANK-R MYXV constructs in New Zealand White (NZW) rabbits

Day	vMyx-WT and Revertants	vMyx-MT5KO	vMyx-148–150KO	vMyx-ANKsKO
0	Inoculation of two rabbits intradermally with 1,000 PFU or FFU for each of the following viruses: vMyx-WT Lausanne, vMyx-MT5KO Revertant, vMyx 148–150 Revertant, vMyx-ANKsKO Revertant	Inoculation of three rabbits intradermally with 1,000 FFU of vMyx-MT5KO	Inoculation of four rabbits intradermally with 1,000 FFU of vMyx-148–150KO	Inoculation of four rabbits intradermally with 1,000 FFU of vMyx-ANKsKO
4	Primary lesions at inoculation sites: 2.0–3.0 cm (8/8) Raised, soft, red lesions (4/8) Raised, red, necrotic lesions (4/8) Multiple secondary lesions on ears (5/8)	Primary lesions at inoculation sites: Raised, soft, red (ca 2.0–2.5 cm) (3/3)	Primary lesions at inoculation sites: Raised, soft, red (ca 2.0–2.5 cm) (4/4) One rabbit exhibited minute secondary lesions on the ear	Primary lesions at inoculation sites: Raised, soft, red (ca. 1.5–2.5 cm) (4/4)
7	Primary lesions red, swollen, necrotic (4.0–5.0 cm) 8/8 Multiple secondary myxomas in the eyes, ears, and nose. (8/8) Gram-negative bacteria infections of conjunctivas (7/8)	Primary lesions red, swollen, necrotic, healing (2.0–2.5 cm) (3/3)	Primary lesions red, swollen, necrotic (ca 3.5–5.0 cm) (4/4) Multiple minute secondary myxomas (4/4), gram-negative bacterial infections of conjunctivas (2/4)	Primary lesions red, swollen, necrotic (ca. 1.5–2.0 cm) (4/4)
9–10	Primary lesions red, swollen, necrotic (5.0–6.0 cm) (8/8) Multiple secondary myxomas, turning necrotic, dyspnea, severe infections of respiratory tracts, prostrated and emaciated animals. All rabbits were sacrificed due to the severity of symptoms	Primary lesions swollen, but healing	Primary lesions swollen, but healing Secondary lesions healing (4/4)	Primary lesions swollen, but healing
17		Full regression of primary lesions	Full regression of primary and secondary lesions	Full regression of primary lesions