Fig. 11.
Spike pattern of immature relay neurons is influenced by the EPSC decay kinetics. Current clamp recordings are shown of immature (A) and mature (B) synapses in response to injection of their respective current waveforms. Waveforms were obtained from representative average synaptic responses to 15 stimuli at different frequencies (from left to right: 100, 10 and 1 Hz) before (black traces) and after bath application of 50 μM EGTA-AM (red traces). Relay neuron spiking was assessed at both Vh of −70 mV (middle) and −55 mV (bottom). i: Control. ii: EGTA-AM. C and D: summary of spike probability following current injection waveform at a Vh of −70 mV (C) and −55 mV (D) at immature (i) and mature (ii) synapse. Statistical data for 1 Hz for immature neurons are not available because neurons did not fire action potentials in response to 1-Hz stimulation/injected current while holding at −70 mV. *P < 0.05.