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. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: Hypertension. 2010 Nov 8;56(6):1145–1151. doi: 10.1161/HYPERTENSIONAHA.110.160515

Fig. 3. Role of the CCAAT Box.

Fig. 3

(A) Sequences of various double stranded EMSA probes. CCAAT box regions are indicated in the shaded area. (B) Nuclear extracts from rat RV (7-day hypoxia treated), mouse heart or HL-1 cardiac muscle cells were subjected to EMSA using wild type or mutant Probe #2. (C) Nuclear extracts were subjected to EMSA with wild type or truncated Probe #2. (D) Heart nuclear extracts were subjected to EMSA using 32P-labeled Probe #2 in the presence of excess cold competitor (unlabelled Probe #2). (E) HL-1 cells were transfected with the luciferase construct controlled by the 250 bp proximal region of the wild type (WT) or mutant (Mut) Gata4 promoter. Values represent means ± SEM of the ratio of 250 bp Gata4 promoter-controlled firefly luciferase activity to thymidine kinase (TK) promoter-controlled Renilla luciferase activity (n = 6). *significantly different from the wild type Gata4 promoter. Some images were grouped from different parts of the same gel and such arrangements are indicated by dividing lines.