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. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: Peptides. 2013 Dec 16;52:74–81. doi: 10.1016/j.peptides.2013.12.006

Table 1.

Specific inhibitors do not inhibit enzyme activity.

Inhibitor Class Inhibitor % of Control (N = 3)
Neprilysin SCH (10 μM) 100
Thimet oligopeptidase CFP (10 μM) 102 ± 3
Thimet oligopeptidase CFP (100 μM) 97 ± 2
Neurolysin Pro-Ile (1 mM) 101 ± 2
Prolyl endopeptidase ZPP (10 μM) 96 ± 2
Serine Aprotinin (80 μM) 100
Serine SBTI (100 μM) 101 ± 2
Aspartic acid Pepstatin A (100 μM) 97 ± 1

Inhibitors SCH39370 (SCH), 1-carboxy-3-phenyl-propyl (CFP), Pro-Ile, Z-prolyl prolinal (ZPP), aprotinin, soybean trypsin inhibitor (SBTI) and pepstatin A did not inhibit [125I]-Ang-(1-4) formation compared to control. Activity was assayed in a CSF pool of control and BMX animals (N=10) in the presence of 100 nM Ang-(1-7) and an inhibitor cocktail (AM, BS, CHYM, BSC, LIS) for 120 minutes at 37°C. Assays were repeated three times.