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. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: Ann Biomed Eng. 2010 Apr 2;38(8):2499–2511. doi: 10.1007/s10439-010-0023-5

FIGURE 3.

FIGURE 3

Immunostaining results for astroyctes (a) Astrocytes immunolabeled with antibodies against glial fibrillary acidic protein (GFAP, red) and counterstained with cell nucleus dye DAPI (blue). Primary astrocytes (passage P3–P5) were seeded on the luminal side of the Transwell filter at a density of 5.5 × 103 cells per filter and cultured for 3 days before immunostaining. The purity of isolated astrocytes was >90%. (b) Cytoplasmic labeling (green) of the astrocyte monoculture by Calcein AM. Astrocytes were seeded onto the luminal side of the Transwell filter at a seeding density of 2.75 × 104 cells per filter and grown for 5 days, forming a confluent monolayer on the luminal side of the Transwell filter which was used to perform the permeability measurement. (c) Labeling of GFAP (red) in astrocytes when cocultured with bEnd3 cells at the end of 5th day.