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. 2004 Jun;186(11):3609–3620. doi: 10.1128/JB.186.11.3609-3620.2004

FIG. 2.

FIG. 2.

Growth of the S. meliloti wild type and the S. meliloti sitA mutant strain with dependence on Mn(II) and/or Fe(II) supplementation of the medium. Overnight cultures of the S. meliloti wild-type strain Rm1021 (A) and the S. meliloti sitA mutant Rm1021ΔsitA (B) were washed and diluted in fresh VMM* containing 30 μM Fe(II) (▪), 60 μM Fe(II) (□), 1 μM Mn(II) (▴), or 30 μM Fe(II) + 1 μM Mn(II) (▵). Growth was monitored via optical density at 580 nm (o.d.580). The results are derived from five independent replicates, and the error bars indicate the standard deviations.