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. 2014 Aug 29;70(Pt 9):1117–1126. doi: 10.1107/S2053230X14015507

Figure 1.

Figure 1

The effect of adjusting seed stock concentration. An initial seed stock was made from crystals of a tyrosine kinase grown in 100 mM HEPES pH 7.0, 8%(w/v) PEG 8000, 7%(v/v) ethylene glycol. MMS into The PEGs Suite screen (Qiagen) was performed with an Oyrx8 robot (Douglas Instruments, UK). Sitting drops were set up in Innovaplate SD-2 crystallization plates, with droplets consisting of 300 nl protein solution, 200 nl reservoir solution and 100 nl seed stock. The figures show the effect of seed concentration on the crystallization in a single condition [0.1 M MES pH 6.5, 25%(w/v) PEG 550 monomethyl ether (MME) from The PEGs Suite]. Drop (a) was seeded with undiluted seed stock, (b) with a 1:100 dilution and (c) with a 1:1000 dilution (Marsh, 2008 ▶; Cowan-Jacob, unpublished results).