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. 2014 Aug 29;70(Pt 9):1117–1126. doi: 10.1107/S2053230X14015507

Figure 6.

Figure 6

MMS and cross-seeding between FAB complex H2L6/IL13 and FAB complexes M1295/IL13 and C836/IL13. (a) Microcrystals of H2L6/IL13 [grown in 28%(w/v) PEG 8000, 0.1 M MES pH 6.5] used as seed stock for the MMS crystallization of H2L6/IL13. (b) Optimized crystals of H2L6/IL13 [grown in 20%(w/v) PEG 3350, 0.2 M ammonium citrate pH 5.1] used to generate a seed stock for MMS crystallization of M1295/IL13 and C836/IL13. (c) Crystals of M1295/IL13 obtained after MMS in 20%(w/v) PEG 3350, 0.2 M sodium citrate pH 8.3. (d) Crystals of C836/IL13 obtained by MMS in 20%(w/v) PEG 3350, 0.2 M ammonium citrate. All figures taken from Obmolova et al. (2010 ▶).