| Method |
Vapor diffusion, sitting drop, seeded |
| Plate type |
Intelli-Plate 24-4 (Art Robbins/Hampton Research) |
| Temperature (K) |
277 |
| Protein concentration (mgml1) |
20 |
| Buffer composition of protein solution |
50mM Tris pH 8.0, 50mM NaCl, 1mM DTT |
| Composition of reservoir solution |
0.1M MOPS/HEPESNa pH 7.9, 0.02M sodium formate, 0.02M ammonium acetate, 0.02M trisodium citrate, 0.02M sodium potassium L-tartrate, 0.02M sodium oxamate, 0.02M ammonium acetate, 12.5%(v/v) glycerol, 25%(w/v) PEG 4000 |
| Cryocondition |
1:3, 100% glycerol:reservoir (final concentration 35%) |
| Volume and ratio of drop |
3l drop, 2:1 (protein:reservoir) |
| Volume of reservoir (l) |
300 |
