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. 2014 Aug 27;70(Pt 9):1211–1214. doi: 10.1107/S2053230X1401574X

Table 2. Crystallization.

Method Sitting drop
Plate type Cryschem plate
Temperature (K) 283
Protein concentration (mg ml−1) 3.5
Buffer composition of protein solution 20 mM Tris–HCl pH 7.0, 200 mM NaCl
Composition of reservoir solution 0.1 M Tris buffer pH 7.0, 1.2 M sodium citrate, 0.2 M sodium chloride
Volume and ratio of drop 2 µl (1:1 ratio)
Volume of reservoir (µl) 400