Skip to main content
. 2014 Aug 27;70(Pt 9):1224–1227. doi: 10.1107/S2053230X14014691

Table 2. Crystallization of the C-terminal domain of Par-4.

Method Vapour diffusion, sitting drop
Plate type 96-well
Temperature (°C) 22.15
Protein concentration (mg ml−1) 40
Buffer composition of protein solution 50 mM potassium phosphate pH 4.0
Composition of reservoir solution 37–42% tert-butanol, 100 mM sodium citrate pH 5.9
Volume and ratio of drop 1.4 µl, 1:1.3 protein:crystallization buffer
Volume of reservoir (µl) 70