Table 1. Macromolecule-production information.

Source organism	O. sativa L. japonica
DNA source	mRNA (GenBank BAA77214.1)
Forward primer†	5′-GCGGCAGCCATGGCGTCGGAGAAGCACTTCAAG-3′
Reverse primer‡	5′-GTAGTAGGATCCTTCTTTATTCAAATCTCAGCAGC-3′
Cloning vector	pKM260
Expression vector	pKM260
Expression host	E. coli NiCo21 (DE3)
Complete amino-acid sequence of the construct produced§	MHHHHHHASENLYFQGAMASEKHFKYVILGGGVAAGYAAREFAKQGVKPGELAIISKEAVAPYERPALSKGYLFPQNAARLPGFHVCVGSGGERLLPEWYSEKGIELILSTEIVKADLASKTLTSAVGATFTYEILIIATGSSVIKLSDFGTQGADSNNILYLREVDDADKLVAAIQAKKGGKAVIVGGGYIGLELSAALKINDFDVTMVFPEPWCMPRLFTADIAAFYESYYTNKGVKIVKGTVAVGFDADANGDVTAVNLKNGSVLEADIVGVGVGGRPLTTLFKGQVAEEKGGIKTDAFFETSVPGVYAVGDVATFPMKMYNELRRVEHVDHARKSAEQAVKAIKGKESGESVVEYDYLPYFYSRSFDLGWQFYGDNVGDTILFGDSDPTSAKPKFGSYWIKDGKVLGAFLEGGSPDENKAIAKVAKTQPPVANIEELKKEGLQFASKI

^†The NcoI site is underlined.

^‡The BamHI site is underlined.

^§The His tag and TEV protease cleavage site are underlined.