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| Method | Hanging drop |
| Plate type | 24-well plate |
| Temperature (K) | 298 |
| Protein concentration (mg ml−1) | 10 |
| Buffer composition of protein solution | 10 mM Tris–HCl pH 8.0 |
| Composition of reservoir solution | 100 mM Tris–HCl pH 8.0, 200 mM lithium sulfate, 30%(w/v) PEG 5000 MME |
| Volume and ratio of drop | 1 µl, 1:1 |
| Volume of reservoir (µl) | 500 |
