Table 1. Macromolecule-production information.

Source organism	V. cholerae
DNA source	Genomic DNA
Forward primer†	AGATATACATATGTCACAGAGTAAAAAAGAG
Reverse primer†	CAAGCTTGTCGACAGGCATCTGTCTGCTTAG
Expression vector	pET-30a
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced‡	MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIELTEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCLILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMPLEHHHHHH

^†The NdeI and XhoI restriction sites are underlined.

^‡The His₆ tag and additional residues are underlined.