Table 2. Crystallization.

Method	Vapour diffusion
Plate type for screening	96-well sitting-drop crystallization plate, Axygen
Plate type for optimization	24-well VDX plate, Hampton Research
Temperature (K)	295
Protein concentration (mg ml−1)	15
Buffer composition of protein solution	10 mM Tris–HCl pH 8.0, 100 mM NaCl
Composition of reservoir solution	1.8 M sodium chloride, 0.1 M sodium potassium phosphate pH 6.4
Volume and ratio of drop	2 µl, 1:1
Volume of reservoir (µl)	500