. 2014 Aug 27;70(Pt 9):1264–1267. doi: 10.1107/S2053230X14017154

Table 1. Chicken CD8αα–BF2*0401 production information.

Source organism	Gallus gallus
DNA source	Cloning from cDNA
Forward primer	ACGCATATGGCCCAGGGCCAGCGTAACACG
Reverse primer	CCGCTCGAGGAAGAAGGCGGGTTGTCCCGAG
Cloning vector	pMD18T
Expression vector	pET-21a(+)
Expression host	E. coli
Complete amino-acid sequence of the construct produced
Chicken CD8α	AQGQRNTMEARFLNRNMKHPQEGQPLELECMPFNIDNGVSWIRQDKDGKLHFIVYISPLSRTAFPRNERTSSQFEGSKQGSSFRLVVKNFRAQDQGTYFCIANINQMLYFSSGQPAFF
BF2*0401	ELHTLRYIRTAMTDPGPGQPWFVTVGYVDGELFVHYNSTARRYVPRTEWIAANTDQQYWDGQTQIGQLNEQINRENLGIRQRRYNQTGGSHTVQWMFGCDILEDGTIRGYRQSAYDGRDFIALDKDMKTFTAAVPEAVPTKRKWEEESEPERWKNYLEETCVEWLRRYVEYGKAELGRRERPEVRVWGKEADGILTLSCRAHGFYPRPIVVSWLKDGAVRGQDAHSGGIVPNGDGTYHTWVTIDAQPGDGDKYQCRVEHASLPQPGLYSW
Chicken β ₂m	DLTPKVQVYSRFPASAGTKNVLNCFAAGFHPPKISITLMKDGVPMEGAQYSDMSFNDDWTFQRLVHADFTPSSGSTYACKVEHETLKEPQVYKWDPEF