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. 2014 Aug 27;70(Pt 9):1264–1267. doi: 10.1107/S2053230X14017154

Table 2. Crystallization conditions.

Method Sitting-drop vapour diffusion
Plate type VDX plate
Temperature (K) 291
Protein concentration (mg ml−1) 5, 10
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 50 mM NaCl
Composition of reservoir solution 0.2 M ammonium sulfate, 0.1 M Tris pH 8.5, 25%(w/v) polyethylene glycol 3350
Volume and ratio of drop 1:1
Volume of reservoir (µl) 160