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| Method | Microbatch-under-oil |
| Plate type | 96-well U-bottom plate |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | ∼12 |
| Buffer composition of protein solution | 20 mM Tris–HCl pH 8.0, 200 mM NaCl |
| Composition of reservoir solution | 40 mM KH2PO4, 15% glycerol, 12%(w/v) polyethylene glycol (PEG) 8000 |
| Volume and ratio of drop | 4 µl (1:1) |
| Volume of reservoir | 0 (microbatch) |
